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Laccase activities were measured using 190 µl of reaction mixtures (100 mM citrate–phosphate buffer pH 4.0 and 1 mM ABTS) in the presence and the absence of a gradient of NaCl (5–190 mM) and 10 µl of purified LacAnc100 (0.34 µg ml −1 ) or LaccID (0.40 µg ml −1 ) in flat-bottom 96-well plates (Greiner Bio-One, 655101)