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Then, the mRNA levels of sterol regulatory element-binding protein 1c (Srebp1c), carbohydrate-responsive element-binding protein (Chrebp), Fas, carnitine palmitoyl transferase I (Cpt1), Pparα, peroxisome proliferator-activated receptor-γ coactivator 1-α (Pgc1α), carnitine O-octanoyl transferase (Crot), 3-hydroxy-3-methylglutaryl-CoA synthase 2 (Hmgcs2), acyl-CoA oxidase 1 (Acox1), Foxa1, fatty acid transport protein 2 (Fatp2), Pparγ, and Cyp2e1 were measured by real-time PCR with Fast SYBR Green Master Mix (Thermo Fisher Scientific) on a Bio-Rad CFX system